Ch. 8- Gene Transfers - Chapter 8 Bacterial Genetics Gene...

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Chapter 8 Bacterial Genetics Gene Transfer 1
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Mechanisms of Gene Transfer s Terms to know: s Recombinants s Homologous recombination s DNA mediated transformation s Transduction s Conjugation s Transposition 2
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Recombinants: having a combination of properties from two cells Strp R Leu - Strp S Leu + or Streptomycin + sugar and salts only Strp R Leu - Strp S Leu + + Streptomycin + sugar and salts only Strp R Leu + 3
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Homologous Recombination: integration of DNA from an outside source only if there is a similar region in the recipient’s genome 4
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DNA-Mediated Transformation s Uptake of “naked” DNA s Natural competence s Entry of ssDNA s Integration of donor DNA by homologous recombination s Multiplication by selection 5
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Fig. 08.19 6
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Fig. 08.19b 7
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DNA-Mediated Transformation s Artificial competence Ex: Escherichia coli Single and double stranded DNA can be introduced Fig. 08.15 8
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s Transfer of DNA by a bacterial virus (bacteriophage)
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This note was uploaded on 06/11/2010 for the course MIBO 2500 taught by Professor Walker during the Spring '08 term at University of Georgia Athens.

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Ch. 8- Gene Transfers - Chapter 8 Bacterial Genetics Gene...

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