Handout 1,2 - MCB 252 Cell Biology, Spring 2008 Handout 01...

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MCB 252 Cell Biology, Spring 2008 Handout 01 Welcome to MCB 252 Cell Biology
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MCB 252 Cell Biology, Spring 2008 Handout 01 • Protein Homeostasis The cell periphery Distribution of the genetic information. The plasma membrane, cell surface receptors and signaling. • Organization of the cell nucleus on the cell nucleus, RNA transcription and processing, nuclear pores, lamina, NLS and NES. on translation and protein structure. on bio-membranes and signaling
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MCB 252 Cell Biology, Spring 2008 Handout 01 Culture of mammalian fibroblasts •Techniques •Experiments Text book Website MCB 253 Text book Discussion sessions
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MCB 252 Cell Biology, Spring 2008 Handout 01 Mouse 3T3 fibroblast Nucleus Cytoplasm Mouse 3T3 fibroblast
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MCB 252 Cell Biology, Spring 2008 Handout 01 Cells in culture Provide homogeneous population of cells. Experimental conditions can be precisely controlled. To grow in culture, animal cells must be provided with a liquid mixture that includes nutrients, vitamins and growth factors .
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MCB 252 Cell Biology, Spring 2008 Handout 01 Signal Reception Amplification Transmission Activation/ repression Response
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MCB 252 Cell Biology, Spring 2008 Handout 01 Main objective: To reach an integrated view of the various cellular levels that regulate gene expression. How is a signal received and transmitted? Biomembranes Structure Properties Membrane receptors and channels
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MCB 252 Cell Biology, Spring 2008 Handout 01 All biomembranes share the same basic structural organization of a phospholipid bilayer in which are inserted other molecules such as cholesterol , glycolipids and proteins . Phospholipid bilayers of biomembranes are semi-permeable , asymmetric, and fluid . • Biomembranes: structure and properties
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MCB 252 Cell Biology, Spring 2008 Handout 01 Small uncharged molecules O 2 , CO 2 , H 2 O , N 2 , Ethanol, Glycerol, Testosterone Large polar molecules and ions Glucose, amino acids (peptides), H + , Na + , Ca 2+ , Cl - • Biomembranes: structure and properties Phospholipid bilayer are semi-permeable
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MCB 252 Cell Biology, Spring 2008 Handout 01 • Biomembranes: structure and properties biomembranes are asymmetric
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This note was uploaded on 06/12/2010 for the course MCB 252 taught by Professor Prasanth during the Fall '08 term at University of Illinois at Urbana–Champaign.

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Handout 1,2 - MCB 252 Cell Biology, Spring 2008 Handout 01...

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