Handout 8 - Chromosome Remodeling

Handout 8 - Chromosome Remodeling -...

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MCB 252 Cell Biology, Spring 2008 Handout 08 N Heterochromatin Euchromatin • Regulation of transcription: Chromatin organization. ..
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MCB 252 Cell Biology, Spring 2008 Handout 08 The two chromosomes 7 of a  human lymphocyte. • Regulation of transcription: Chromatin organization. .. During interphase, chromosomes occupy distinct domains of the nucleus,  referred to as “Chromosome territories”. Chromosomes are visualized  by in situ hybridization.  
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MCB 252 Cell Biology, Spring 2008 Handout 08 Chromosome 7 Probes • Regulation of transcription: Chromatin organization. .. 1.  Labeled probes  corresponding to the entire  chromosome 7 are produced.
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MCB 252 Cell Biology, Spring 2008 Handout 08 Chromosome 7 Probes • Regulation of transcription: Chromatin organization. .. 2.  Cells are fixed, DNA  denatured, and the labeled probes  are directly  applied to the  samples, hence the name “in situ  hybridization” .
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MCB 252 Cell Biology, Spring 2008 Handout 08 • Regulation of transcription: Chromatin organization. .. This technique is called: “Chromosome painting”
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MCB 252 Cell Biology, Spring 2008 Handout 08 Chromatin remodeling CLOSED  state chromatin “Condensed” OPEN  state chromatin “Decondensed” ? • Regulation of transcription: Chromatin dynamics
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Handout 08 • Regulation of transcription: Chromatin dynamics Genetic links between gene activation and chromatin: 1.   Nucleosome depletion in vivo was found to result in transcriptional  activation in a general manner.  This result suggested that removal of  nucleosomes is coupled to gene activation. 2.
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Handout 8 - Chromosome Remodeling -...

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