Handout 4 - Properties of Actin - PropertiesofActin Ac tina...

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Actin Cytoskeleton Properties of Actin Ac tin a s s e m b ly Ro le  o f ATP Tre a dm illing Role of actin in cell movement Ac tin is  re q uire d fo r m o ve m e nt Ac tin a s s e m b ly c a n drive  m o ve m e nt Regulation of actin assembly in cells Pro te ins  invo vle d in a c tin a s s e m b ly/dis a s s e m b ly Turning  the  pro te in invo vle d in a s s e m b ly a nd  dis a s s e m b ly O N a nd O FF Building stable actin structures
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The cytoskeleton: early studies  Electron microscopy defined 3 types of filaments in cells (1950s- 60s). 1)  Microfilaments : thinnest actin polymers 2)  Intermediate   Filaments : various (lamins) 3)  Microtubules : tubulin  The subunits comprising these filaments were purified  biochemically (late ‘60s- early ‘70s)  Antibodies against the components were used in fluorescence  microscopy to examine filament structure in the whole cell
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Cytoskeletal polymers Pollard and Earnshaw (35-2) microfilaments intermediate filaments microtubules
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Actin: stable structures and dynamic structures
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Dynamic actin is regulated by signals and protein cascades
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G-Actin Monomers  42kDa globular protein monomer of microfilaments One of the most abundant intracellular proteins in eukaryotes (10% of  muscle protein).  Highly conserved throughout evolution with ~90% identity between  widely divergent species from flies to humans.  Different isoforms have different specialized functions (humans have six 
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This note was uploaded on 06/12/2010 for the course MCB 252 taught by Professor Prasanth during the Fall '08 term at University of Illinois at Urbana–Champaign.

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Handout 4 - Properties of Actin - PropertiesofActin Ac tina...

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