Handout 10 - Microtubules - Cytoskeleton Microtubules

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
Cytoskeleton Microtubules Structure of monomer/polymer Dynamics of assembly Dynamic instability Microtubule-associated proteins (MAPs) Microtubule organizing centers (MTOCs)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Microtubule-based structures ciliated epithelium (oviduct) flagellum mitotic spindle Paramecium
Background image of page 2
Microtubule-organizing centers   (MTOCs) Centrosome  a pair of centrioles surrounded by pericentriolar material (PCM) Centrioles Pollard and Earnshaw, Fig.  37-9 EM (two orthogonal centrioles) One centriole - 9 triplet MTs
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Centrosome and Spindle Poles
Background image of page 4
Basal bodies
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
In vivo imaging of mitotic  spindles in  Drosophila  embryos Express GFP-Tau fusion protein in embryos;  visualize with confocal microscopy Dr. Andrea Brand, Cambridge University, UK Movie:  06_cell_divisions.avi 
Background image of page 6
Microtubules First defined by EM in 1963 Assembled from heterodimers of alpha- and beta- tubulin. Dimers are stable and rarely dissociate from another. alpha- and beta-tubulin ~40% identical residues Tubulins are highly conserved: >75% identical  between plants and animals
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Structure of tubulin dimer Lodish, Fig.  18-3 non-exchangeable exchangeable
Background image of page 8
Structure of tubulin polymer Lodish, Fig. 18-3
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 06/12/2010 for the course MCB 252 taught by Professor Prasanth during the Fall '08 term at University of Illinois at Urbana–Champaign.

Page1 / 31

Handout 10 - Microtubules - Cytoskeleton Microtubules

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online