Handout 12 - MT Motor Proteins (Kinesins)

Handout 12 - MT Motor Proteins (Kinesins) - M CB 252 Par t...

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MCB 252 Part 2 MT Motor Proteins
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Kinesin- and dynein-mediated transport Lodish, Fig. 18-27 Kinesins = plus end directed motors Dyneins = minus end directed motors
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MT-based motility Axonal transport: Newly synthesized proteins must be moved from cell body to tip of axon (anterograde). Old membranes must be transported back (retrograde) Remember! Axons can be up to 1 meter long. Lodish, Fig. 18-5
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Experiment: Axonal Transport (Pulse-Chase) Different materials move at different speeds fast: 0.5-2.5 μm/sec slow: 10-100 nm/sec So, round-trip from spinal cord to foot, takes 3 weeks to 3 months Lodish, Fig. 18-17
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Visualizing axonal transport Squid ( Loligo ) Prof. George Langford, Dartmouth Univ. dissect giant axon Alberts, Panel 11-3 microscopy Dr. Ron Vale, UCSF
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Bidirectional movement on MTs Lodish, Fig. 18-18
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Vesicles + ATP + MTs = no movement (because no motors present) “Above” + squid cytoplasm extract = movement (because motors present) Substitute non-hydrolyzable ATP analog: no movement
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This note was uploaded on 06/12/2010 for the course MCB 252 taught by Professor Prasanth during the Fall '08 term at University of Illinois at Urbana–Champaign.

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Handout 12 - MT Motor Proteins (Kinesins) - M CB 252 Par t...

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