Handout 16 - Cell Junctions - CellJunctions Tightjunctions...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
Tight junctions Gap junctions For Each Description What do they do? What are they made of? What happens when they don’t work?   Cell Junctions
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Structures at cell/cell or cell/ECM junctions Lodish, Fig. 6-5 Desmosomal cadherins, Desmoplakin and plakoglobin E-cadherin, catenins Adhesion belt
Background image of page 2
Polarity of epithelial cells Alberts, et al. Fig. 19-23 see Lodish 11.6 (Fig. 11-29) for details of  glucose transport from intestinal lumen to  blood apical surface≠ basolateral surface Tight junctions (TJs):  prevent diffusion  maintain polarity  selective transport (Transcellular  transport)
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
EM views of tight junctions Lodish, et al. Figs. 19-9, 19-15 Focal connections
Background image of page 4
Freeze-fracture EM: Tight Junctions Lodish, et al. Fig 19-15 TJ strands (between two epithelial cells) Honeycomb-like networks of ridges and grooves (sealing strands)  Freeze-fracture EM: exposes proteins within the lipid bilayer 
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Tight Junctions Alberts 19-26
Background image of page 6
Transcellular vs. paracellular transport  Lodish, et al. Fig. 19-17 (uptake on one side and  release on the other) (molecules move extracellularly  through parts of TJs) ions, water, small molecules  Control of flows of solutes !
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Role of tight junctions in plasma membrane Liposomes (phospholipid bilayer vesicles, made in  vitro) Fuse 2 different types of liposomes to apical surface  of MDCK cells: 1: fluorescent-labeled outer leaflet 2: fluorescent-labeled inner leaflet or 1 2 apical + basal apical + basal apical + basal –Ca 2+ –Ca 2+ fuse fuse apical signal MDCK cell
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 06/12/2010 for the course MCB 252 taught by Professor Prasanth during the Fall '08 term at University of Illinois at Urbana–Champaign.

Page1 / 20

Handout 16 - Cell Junctions - CellJunctions Tightjunctions...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online