Handout 17 - Embryonic Cell Cycle and Biochemical Analysis

Handout 17 - Embryonic Cell Cycle and Biochemical Analysis...

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Unformatted text preview: Cell cycle I Embryonic cell cycle; Biochemical analysis Basic Features Cell fusion experiments Embryonic cell cycles Xenopus (biochemical analysis) Identification of MPF Identification of Cyclins The Eukaryotic Cell Cycle Alberts, et al. Fig 17-13 Flow cytometry to analyze DNA content Fluorescence-activated cell sorter (FACS) Lodish, et al. Fig. 9-28 Label cells with a DNA dye Cell fusion experiments Fuse cells together, monitor effects S G1 + G1 nucleus enters S S G2 + G2 nucleus does not enter S S phase cells have factor that can induce DNA replication in G1 cells G2 cells do not respond to this factor. Block to re-replication: replicate once and only once Fuse mitotic cells w/ interphase cells In all cases, the nuclear envelope breaks down and chromosomes condense. Pollard and Earnshaw, Fig. 46-4; see Lodish Fig. 20-3 Advantages of studying embryonic cell cycle- In embryos, cycles are synchronized- Early cycles are rapid because frogs make swimming tadpoles as fast as they can to not get...
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This note was uploaded on 06/12/2010 for the course MCB 252 taught by Professor Prasanth during the Fall '08 term at University of Illinois at Urbana–Champaign.

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Handout 17 - Embryonic Cell Cycle and Biochemical Analysis...

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