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Handout 17 - Embryonic Cell Cycle and Biochemical Analysis

Handout 17 - Embryonic Cell Cycle and Biochemical Analysis...

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Cell cycle I Embryonic cell cycle; Biochemical analysis Basic Features Cell fusion experiments Embryonic cell cycles Xenopus (biochemical analysis) Identification of MPF Identification of Cyclins
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The Eukaryotic Cell Cycle
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Alberts, et al. Fig 17-13 Flow cytometry to analyze DNA content Fluorescence-activated cell sorter (FACS) Lodish, et al. Fig. 9-28 Label cells with a DNA dye
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Cell fusion experiments Fuse cells together, monitor effects S G1 + G1 nucleus enters S S G2 + G2 nucleus does not enter S S phase cells have factor  that can induce DNA  replication in G1 cells G2 cells do not respond  to this factor. Block to  re-replication: replicate  once and only once
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Fuse mitotic cells w/ interphase cells In all cases, the nuclear envelope breaks down and chromosomes condense.   Pollard and Earnshaw, Fig. 46-4;  see Lodish Fig. 20-3
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Advantages of studying  embryonic cell cycle - In embryos, cycles are synchronized
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