Handout 19

Handout 19 - CDKs (Cyclin-dependent kinases) Required for...

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CDKs (Cyclin-dependent kinases) Cyclins determine target specificity of the CDK Required for Mitosis Required for Replication, S-phase Required for Start, Centrosome duplication
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Activity of Budding Yeast CDKs 9 Cyclins which form 9 CDKs: Cln3, Cln1, Cln2, Clb5, Clb6, Clb3, Clb4, Clb1, Clb2 Different Cdks turned on and off at distinct times in the cell cycle through a variety of regulatory switches.
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Overview of Regulation Degrade S- and M- cyclins Degrade G1 cyclins START Wrong Inactivate CDKs by degrading distinct classes of cyclins
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Overview of Cdk Regulation Activation of Early S-Cdk START Some CDKs activated by cyclin production: 1, 2, 3 Some CDKs initially inactive: 4, 5 1 2 3 4 5 Produce S-phase cyclins but keep the S-Cdk in an inactive form Activation of M-Cdk Produce M-phase cyclins but keep the M-Cdk in an inactive form
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How is cyclin degraded? 90 N-terminal amino acids - Cyclin is not degraded ( 13 is degraded) Attach Cyclin residues 13-91 to different protein that’s not normally degraded. This fused protein is now degraded in mitotic extracts but stable in
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Handout 19 - CDKs (Cyclin-dependent kinases) Required for...

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