Handout 2 - MC B2 5 2 C e lls &T is s ue s Fa ll2 0 0 9...

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    Ha ndo ut 2 I. The Cellular Periphery and Its Role in Controlling Gene Expression a. Physiology of the Plasma Membrane a. Carriers and channels of the plasma membrane b. Cell surface receptors c. Reception and Integration of signals I d. Reception and Integration of signals II I. Transcriptional Control of Gene Expression II. Translational Control of Gene Expression
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    Ha ndo ut 2
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    Ha ndo ut 2 Ho w is  a  s ig na l re c e ive d a nd tra ns m itte d b y a   c e ll? I. a . Phys io lo g y o f the  Pla s m a  Me m b ra ne
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    Ha ndo ut 2 I. a . Phys io lo g y o f the  Pla s m a  Me m b ra ne Pho s pho lipids  a re  a m phipa thic  o r a m phiphillic
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    Ha ndo ut 2
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    Ha ndo ut 2 1. S pe c ific  lipid c o m po ne nts  o f the  m e m b ra ne  a re  dis trib ute d  a s ym m e tric a lly b e twe e n the  two  le a fle ts  o f m e m b ra ne s : flip- flo p m e c ha nis m I. a . Phys io lo g y o f the  Pla s m a  Me m b ra ne (Exoplasmic leaflet) (Cytosolic leaflet) 2. Pho s pa tidyls e rine  a nd a po pto s is
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    Ha ndo ut 2 I. a . Phys io lo g y o f the  Pla s m a  Me m b ra ne Fluidity Fluidity o f the  lipid b ila ye r de pe nds  o n b o th its  c o m po s itio n a nd te m pe ra ture  
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    Ha ndo ut 2 Labeling of membrane proteins with a Fluorescent reagent Photobleaching of a small area Fluorescence recovery overtime intensity time (s) F luorescence R ecovery A fter P hotobleaching FRAP Viewed by fluorescence microscopy
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    Ha ndo ut 2 I. a . Phys io lo g y o f the  Pla s m a  Me m b ra ne
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    Handout 2 • Biomembranes: structure and properties S o m e  m e m b ra ne  pro te ins  a re  m a de  le s s  m o b ile  b y inte ra c tio n with  e le m e nts  o f the  c yto s ke le to n o r o f the  e xtra -c e llula r  matrix
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    Handout 2 Cell can confine proteins and lipids to specific regions of  the plasma membrane
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Handout 2 - MC B2 5 2 C e lls &T is s ue s Fa ll2 0 0 9...

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