FAMEs Lab - Gas Chromatography of Fatty Acid Methyl Esters...

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Gas Chromatography of Fatty Acid Methyl Esters (FAMES) John Goodson Partner(s) : Jessica Highsmith Date Performed: 1 April 2010
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Abstract: The purpose of this study was to perform an analysis of the fatty acid composition of edible oils using gas chromatography. This particular group focused its study on pecan oil. Introduction: Gas chromatography is used to separate volatile components of a mixture. An inert gas is used to convey the sample, as a vapor, through a column consisting of an inert support and a stationary phase that interacts with sample components, thus it is a type of partition chromatography. The technique uses very narrow fused silica tubes that have stationary phase films bonded to the surface. The ability of the film to retard specific compounds is used to separate compounds of differing polarity within the column. The column is placed in an oven that has exceptional temperature control, and heated to provide separation of wide-boiling range compounds. The carrier gas is usually hydrogen or helium (in this experiment helium), and the eluting compounds can be detected several ways, including flame ionization detectors, by changes in properties of the carrier (thermal conductivity detector), by mass spectrometry, and other methods. Quantitative analysis of the compounds can then be carried out but comparison of either the area or height of the analyte peaks with that of one or more standards. In this manner, we can identify compounds in the original sample with comparison to known response factors and relative response factors.
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This note was uploaded on 06/15/2010 for the course FDST 4080 taught by Professor Pegg during the Spring '10 term at Georgia Southern University .

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FAMEs Lab - Gas Chromatography of Fatty Acid Methyl Esters...

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