quiz2key - Mic102/Spr08/Appleman Quiz 2, 30 April 2008 This...

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Mic102/Spr08/Appleman Quiz 2, 30 April 2008 This quiz is to represent only your own work . As a UC Davis student, you are bound by an honor code; if you can’t be trusted with that, can you be trusted in a career in, say, the health professions? Not only does this honor code demand personal integrity, but it also requires you to demand integrity from your peers. This quiz is closed book, closed note. Please hand in a blue scantron with the answers to the multiple choice section of the quiz. Make sure that it has your name, student I.D.#, and “ Test form B ” filled in. You will also need to hand in the last two pages of this quiz, with your name on each. Most of what we know about biology we have learned by the study of model organisms. Once we have thoroughly understood these representatives of a group, we are well equipped to make reasonable guesses about how similar organisms will behave. One of the foremost model organisms is Rhodobacter sphaeroides , a member of the Bacteria. It is capable of many different metabolic lifestyles, and is easy to grow in the lab. It has been used to study such processes as autotrophy, phototrophy, and respiration. Not surprisingly, it has a genome that is much larger than the bare minimum of about 300 genes—it has over 4000 genes. It is a common bacteria in freshwater, where it is commonly found as a scum on the bottom of shallow ponds. Sure, it’s pond scum—but it is pond scum with talents and capabilities far beyond our own!
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1. Under both aerobic and anaerobic conditions, R. sphaeroides is capable of fermenting glucose to a mixture of acetate, CO 2 , H 2 , and lactate. Why would it use fermentation under these conditions? a) it extracts the most energy from a molecule of glucose b) it is extremely fast c) it permits autotrophic growth d) it is the only way to get energy under anaerobic conditions 2. Which of these pathways is R. sphaeroides using in the above fermentation? a) the Entner-Doudoroff pathway— limited to Anaerobes b) glycolysis c) β -oxidation— for fatty acids, not glucose d) all of the above 3. When R. sphaeroides is fermenting under anaerobic conditions, its growth can be assisted by the presence of methanogens. How? a) the methanogens consume O 2 , making fermentation more efficient b) the methanogens produce reduced carbon, which can be used for fermentation c) the methanogens consume H 2 , increasing the energy yield from the fermentation- - Δ G (actual) is a function of concentrations of products and reactants d) the methnaogens neutralize acetate, making the pH optimal for growth 4. R. sphaeroides can also respire aerobically, using a wide variety of organic compounds as electron sources and giving off CO 2 . How do electrons from organic compounds enter the R. sphaeroides aerobic electron transport chain? a) NADH and FADH from the Krebs cycle
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This note was uploaded on 06/15/2010 for the course MIC MIC 102 taught by Professor Alexappleman during the Spring '08 term at UC Davis.

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quiz2key - Mic102/Spr08/Appleman Quiz 2, 30 April 2008 This...

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