last hw assignment KEY

last hw assignment KEY - 1 of 4 Biological Sciences 102...

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1 of 4 Biological Sciences 102 Answer Key Winter, 2010 M. Etzler, K. Hilt Last Homework Assignment Assigned text problems: Biochemical Calculations (Segel) 4-4 on pp. 223-224 and 4-9 on pp. 237-238 pp. 319 #2, 3, 6, 10, and 12. You need to be able to plot and interpret Lineweaver-Burk graphs. 1. Enzyme Z catalyzes the reaction: A + NAD + B + NADH + H + Besides following the [H + ] using a pH meter, the reaction can be monitored spectroscopically at 340 nm. This is the wavelength at which NADH absorbs light. Some of the first enzymes that were worked on in biochemistry were dehydrogenases, like our enzyme Z, because of this advantage. Radioisotopes were not yet available. a) Diagram what the kinetic assay results would be if the reaction was measured from left to right. What would it be if the assay was measured from right to left? [NADH] left to right (red line) r i g h t t o l e f t (green line) Time b) Describe how you would accurately determine enzyme Z's K m for NAD + . Describe the experimental set-up and the analysis of data. Assay a series of tubes where the concentrations of buffer, compound A, and enzyme Z were constant. Vary the concentration of NAD + . Make a Lineweaver-Burk plot and determine the K m from the x-intercept: 1 / v o 1/[S] c) Imagine that twenty I.U.’s of enzyme Z were catalyzing the above reaction for one minute,
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This note was uploaded on 06/23/2010 for the course BIS 102 taught by Professor Hilt during the Winter '08 term at UC Davis.

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last hw assignment KEY - 1 of 4 Biological Sciences 102...

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