Assignment 4

Assignment 4 - Solution for Assignment 4 - Segel book,...

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Solution for Assignment 4 - Segel book, Pages 143 – 144. Problem 1) Since a cation exchange resin was used, as you learned in class, the amino acids will elute from the column in the order of their isoelectric points. Thus, you would expect to have the following order: Glutamate, Serine, Tryptophan, Alanine, Lysine. Segel changed the order to account for the hydrophobicity of some of of the amino acids as he explains in his answer at the back of the book. As stated in class, you are not held account for recognizing this degree of hydrophobicity at the present time. Problem 2) The answer at the back of the book explains it adequately.
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Problem 3) Part a tells you the amino acids that are present in the peptide but not the order in which they appear. From the way it is worded you can assume that each of those amino acids is present once. Part b tells you that lysine is the only amino acid that is labeled at its alpha
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Assignment 4 - Solution for Assignment 4 - Segel book,...

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