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Old Midterm 2 - PLANT BIOLOGY 113 SPRING 2008 MIDTERM#2...

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P LANT B IOLOGY 113, S PRING 2008 M IDTERM #2 P AGE 1 M AY 22, 2008 NAME MIDTERM #2 - 300 POINTS TOTAL. This is a closed book exam due at the end of class. You may use notes handwritten on one side of a 4" x 6" card. Put your name on the card and turn it in with your test. Please be sure that you have 6 pages. Good luck! QUESTION 1 (20 POINTS) : List two ways in which expression of the T-DNA opine synthase gene in a crown gall tumor benefits the bacteria. QUESTION 2 (25 POINTS) : Predict whether or not the T-strand will be formed during the infection process with Agrobacterium tumefaciens with a loss-of-function mutation in the following Ti plasmid genes. Briefly justify your answer. virA mutation: virB mutation: virD2 mutation: virE2 mutation: tms mutation:
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P LANT B IOLOGY 113, S PRING 2008 M IDTERM #2 P AGE 2 M AY 22, 2008 NAME 3. You have isolated DNA fragments containing components of the wild type T-DNA, such as the left border and TMS locus. Each component is inserted into the indicated sites in T-DNA #1 and T-DNA #2. The modified T-DNAs are then transferred into plants. LB NPT II RB SITE RB T-DNA #1 LB TMS SITE OPINE SYNTHESIS RB T-DNA #2 QUESTION 3A (25 POINTS) : Why is the NPT II gene often inserted into the T-DNA? How is this bacterial gene expressed in plant cells?
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