Old Midterm1

Old Midterm1 - PLANT BIOLOGY 113, SPRING 2008 APRIL 24,...

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PLANT BIOLOGY 113, SPRING 2008 MIDTERM #1 PAGE 1 APRIL 24, 2008 NAME HARD COPY OF ANSWERS DUE BY 5 PM, FRIDAY, APRIL 25 IN THE PLANT BIOLOGY OFFICE, 1002 LS. You can use your notes, handouts, and text only. You are operating under the UC Davis Honor Code - DO NOT CONSULT WITH ANYONE ABOUT THIS EXAM. Please be sure that you have 6 pages. Some of the examples are hypothetical. 300 POINTS TOTAL QUESTION 1 (15 POINTS) : What is a gametophyte? What is a major difference between a gametophyte and a sporophyte? Name the two gametophytes of plants. QUESTION 2 (15 POINTS) : List two similarities and two differences between the molecular definition and the genetic definition of a gene. Question 3. You are conducting an undergraduate research project characterizing the organization of a newly discovered plant, Selaginella obamaceae . E XPERIMENT A: You prepare a genomic library consisting of 1 kb fragments from S. obamaceae genome. You then pick 100 clones at random, isolate the 1 kb genomic DNA fragments from these clones, label the fragments with radioactivity, and hybridize each fragment individually with a gel blot of nuclear DNA from S. obamaceae . The following results are obtained. HYBRIDIZATION WITH 1 KB GENOMIC DNA FRAGMENTS 60 clones with pattern A 40 clones with pattern B (NOTE: clones generate similar but not identical patterns) QUESTION 3A (20 POINTS) : On the basis of this experiment alone, what fraction of the S. obamaceae genome consists of repeated and single (or low) copy DNA? Explain and qualify your answer.
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PLANT BIOLOGY 113, SPRING 2008 MIDTERM #1 PAGE 2 APRIL 24, 2008 NAME E XPERIMENT B: Next, you prepare a genomic library that consists of 20 kb fragments of the
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Old Midterm1 - PLANT BIOLOGY 113, SPRING 2008 APRIL 24,...

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