Lecture 2

Lecture 2 - Lecture 2: How are cells studied? What do cells...

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How are cells studied? What do cells do? Cell composition? Peripheral sensory nerves (PSNs) - Neurons - Schwann cells (glial cells) Endothelial cells (Ecs) – single cell layer that lines the vascular system - Arteries, veins - Vasculogenesis angiogenesis (remodeling) Why the Mukouyama article? - All follow rules of evidence. - Use about 10-12 basic cellular and biology techniques - Hypothesize: Is there some sort of correlation? (plausibility) o Independent control o Nerves organize what blood vessels do (particularly arteries) o Blood vessels develop first and organize surrounding tissue - Try to determine mechanism of action (gain of function/ loss of function) - Hypothesis: Peripheral Sensory Nerves are necessary for arteriogenesis . Gestation period of mouse: 21-22 days Worked with mouse embryo limb skins (E9-E15.5) E stands for day # Techniques used for Figures 1-5 1. IHC (Immunohistochemistry) - (use specific antibodies directed on cells/tissue) 2. Fluorescence microscopy (labeled antibodies) How are cells studied? A. The Scientific method and evidence based hypothesis testing B. Two classes of information a. Observation: What happens/When? b. Experimentation: How does it happen?/Discovering mechanisms and casual
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Lecture 2 - Lecture 2: How are cells studied? What do cells...

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