BIS 104 immuno concepts spr 2010

BIS 104 immuno concepts spr 2010 - (fig 18.26 How does...

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Terminology Antigen = immunogen Epitope = determinant Immunodominant epitope Process Immunization Inject antigen (+ adjuvant): IV; IP; IM multiple times Antigen is processed and presented by macrophages and antigen- presenting cells (APCs) Presented to surface receptors on B-cells (Karp, p. 697 – 700) Antigen recognition by B-cells; receptor mediated activation Clonal expansion of B-cells B memory cells Plasma (Ig) secreting cells Synthesis and secretion of immunoglobulins (Ig) = antibody Multiple forms: IgG; IgM Multivalent Collect and characterize antibody Whole blood Serum (cells and clotting factors removed) Fractionate out IgG from other proteins (albumin)
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Obtain specific antibody by affinity chromatography or similar means
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Unformatted text preview: (fig 18.26) How does antibody react with antigen? Immunoprecipitation Agglutination Block, mask, or inhibit function How to identify and locate antigen In solution In / on cells (immunocytochemistry) In / on tissues (immunohistochemistry) On western blots (immunoblotting) Visualization of antigen – antibody interaction Label, tag antibody Radio-isotope ( 125 I; 3 H; 32 P; 35 S) (table 18.1; figs 18.20, 21) Fluorochrome (fluorescein isothyocyanate; FITC) (fig 1.12) Enzyme (horseradish peroxidase: HRP) Colloidal gold or ferritin (for immuno-electron microscopy) (fig 8.23; 9.40) Production of mono-clonal antibodies (mabs): see Karp, p. 774-776 ....
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