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Unformatted text preview: (fig 18.26) How does antibody react with antigen? Immunoprecipitation Agglutination Block, mask, or inhibit function How to identify and locate antigen In solution In / on cells (immunocytochemistry) In / on tissues (immunohistochemistry) On western blots (immunoblotting) Visualization of antigen – antibody interaction Label, tag antibody Radio-isotope ( 125 I; 3 H; 32 P; 35 S) (table 18.1; figs 18.20, 21) Fluorochrome (fluorescein isothyocyanate; FITC) (fig 1.12) Enzyme (horseradish peroxidase: HRP) Colloidal gold or ferritin (for immuno-electron microscopy) (fig 8.23; 9.40) Production of mono-clonal antibodies (mabs): see Karp, p. 774-776 ....
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- Summer '08
- cell biology, Karp, Immunization Inject antigen, multiple times Antigen