3AL-Lecture6 - Chromatography! Separation of Different...

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Chromatography ! Partition ! Adsorption Separation of Different Compounds
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stationary phase (side view) adsorb compounds onto stationary phase mobile phase TIME Adsorption Chromatography Compounds that have Different Interactions with the Mobile Phase Versus the Stationary Phase can be Separated Compound C Stronger interactions with the mobile phase and/or weak interactions with the stationary phase leads to more time moving with the mobile phase. Compound A Stronger interactions with the stationary phase leads to less time moving with the mobile phase. AA A B B B C C C A BB B C C C A C A A B B C C B A B C add solvent A A B B C C C B A
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Stationary Phases Polar Non-Polar Ionic Si O Si O Si O Si O Si O O O H O O O O O O O O H H ! - ! - ! - ! - ! - ! - ! - ! + ! + ! + silica gel alumina Si O Si O Si O Si O Si O O O O O O O O O O O reverse phase S O O O Na S O O O Na Me 3 N Me 3 N Cl Cl cation exchange anion exchange Al O O O O O O Al O O Al O O O O Al O O O O ! - ! - ! - ! - ! - ! - ! -
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Si O Si O Si O Si O Si O O O H O O O O O O O O H H Si O Si O Si O Si O Si O O
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This note was uploaded on 06/28/2010 for the course CHEM Chemistry taught by Professor Nitche during the Spring '08 term at University of California, Berkeley.

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3AL-Lecture6 - Chromatography! Separation of Different...

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