3 - Control 7/1/10 Identification of a large conductance...

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7/1/10 ETHANOL SLO-1 Activation Further Reduction in Transmitter Release K + Ca+ + Ca++ channel Model for the effects of ethanol on neuronal function Egg Laying
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7/1/10 Unique to BK channels Activated by calcium Activated by depolarization (outward rectifier) Large potassium conductance (30 to 250 pS) Blocked by iberiotoxin (IbTX) Characteristics of BK potassium channels No No Yes Yes
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7/1/10 Recording currents from C. elegans neurons in vivo 5 μ m Pdat::gfp
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Click to edit Master subtitle style 7/1/10 Identification of an outward-rectifying current sensitive to the BK-channel blocker iberiotoxin in the C. elegans neuron CEP -60 mV holding potential -150 mV +120 mV 100 nM IbTX
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Unformatted text preview: Control 7/1/10 Identification of a large conductance outwardly rectifying single-channel potassium current Conductance = 38 pS EK Saline asymmetrical symmetrical 7/1/10 Identification of a slo-1-dependent current Incidence of BK single-channel activity: Wild-type 65% of 37 neurons slo-1(eg73) 0% of 12 neurons slo-1(js118) 0% of 12 neurons Wild type slo-1(eg73) slo-1(js118) pA/pF mV slo-1(-) N2 7/1/10 Ethanol increases outward current in wild-type neurons but not in slo-1 neurons wild-type slo-1(eg73) 7/1/10 Ethanol reversibly activates the native SLO-1 channel in isolation Excised patch Recording pipette Neuron Channels...
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3 - Control 7/1/10 Identification of a large conductance...

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