MidtermIIF08A

MidtermIIF08A - BIS 101-003 (Engebrecht) Fall 2008 Name: _...

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BIS 101-003 (Engebrecht) Name: ______________________________ Fall 2008 Last, First ID #:_______________________________ BIS 101-003 - MIDTERM IIA This exam has a total of 100 points. READ THE QUESTIONS CAREFULLY BEFORE YOU ANSWER. SHOW ALL WORK TO OBTAIN FULL CREDIT. Please sign your name on the score sheet. This exam has nine pages including this cover page and the score sheet HONOR CODE: My signature below affirms that I wrote this exam in the spirit of the honor system of the University of California, Davis. I neither received nor furnished any “help” during the exam, nor did I use any unauthorized references. Signature: ______________________________________________________ Good luck Authorization for Public Distribution of Graded Examination I, ______________________________________, ID# _______________________ authorize that after this exam is graded, it can be placed in a holding bin, labeled BIS 101- 003/Engebrecht, on the first floor of Briggs Hall.
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(6pts) In a generalized transduction experiment donor E. coli have the genotype his+ trp+ gal- and recipient cells have the genotype his- trp- gal+. You perform the following two experiments: Experiment Selected marker Unselected markers (co-transduction frequency) 1 his 10% trp+, 30% gal- 2 trp 50% gal-, 10% his+ a) (3pts) Which genes are closest to each other? Why? b) (3pts) What is the order of the three genes? 2. (10pts) You extract total genomic DNA from E. coli , C. elegans and mouse. You perform a denaturation experiment and see the following: a) (3 pts) What does this tell you about these three genomes? b) (3pts) If C. elegans has 32% A, please indicate the % composition of each of the remaining
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This note was uploaded on 07/02/2010 for the course BIS BIS 101 taught by Professor Sanders during the Spring '09 term at UC Davis.

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MidtermIIF08A - BIS 101-003 (Engebrecht) Fall 2008 Name: _...

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