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Unformatted text preview: A. 27% G; 23% A; 27% C; 23% T dsDNA, eukaryote/prokaryote/virus B. 27% G; 27% A; 23% C; 23% U ssRNA virus C. 23% G; 27% A; 27% C; 23% T ssDNA virus D. 27% G; 23% A; 27% C; 23% U dsRNA virus E. You look at the denaturation kinetics of samples A and C and see the following absorbance at 260nm. Please label which line corresponds to sample A and which to sample C. A 260 1.0 65 70 75 80 85 90 95 Temperature ( o C) C A 2. What would be the consequence at the restrictive temperature of a temperature sensitive mutant in polI that completely inactivates the protein? Please draw what a fork would look like in the mutant. Be sure to label your picture with the polarity of the strands and what everything is and indicate the defect. Because polI provides the exonuclease to remove the RNA primers, these will remain in the mutant. Leading strand RNA primer 3’ 5’ 5’ 3 5’ 3 5 Lagging strand Okazaki fragments 3 =...
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- Spring '09
- DNA, tyr slo aro, aro slo tyr, aro- slo+ Experiment