Homework04key - A 27 G 23 A 27 C 23 T dsDNA...

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From Homework03: You are trying to determine the position on the E. coli chromosome of a new mutation that you isolated, called slo- (slow growing). You do generalized P1 transduction experiments using donor and recipient strains as shown below. You observe the following cotransduction frequencies: Donor: Recipient: tyr+ aro+ slo- tyr- aro- slo+ Experiment Selected marker Unselected markers 1 tyr+ 25% aro+, 30% slo- 2 aro+ 22% try+, 80% slo- Give the order of the genes. The order must be: tyr slo aro (or aro slo tyr) 1. You have obtained genome nucleic acid from different sources and have determined the base content. Please identify the genome structure for the following samples. State if the genome is RNA or DNA and if is likely to be single-stranded (ss) or double-stranded (ds). State if it could be from a eukaryote and/or prokaryote and/or virus. Please make sure to give a complete answer to obtain full credit.
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Unformatted text preview: A. 27% G; 23% A; 27% C; 23% T dsDNA, eukaryote/prokaryote/virus B. 27% G; 27% A; 23% C; 23% U ssRNA virus C. 23% G; 27% A; 27% C; 23% T ssDNA virus D. 27% G; 23% A; 27% C; 23% U dsRNA virus E. You look at the denaturation kinetics of samples A and C and see the following absorbance at 260nm. Please label which line corresponds to sample A and which to sample C. A 260 1.0 65 70 75 80 85 90 95 Temperature ( o C) C A 2. What would be the consequence at the restrictive temperature of a temperature sensitive mutant in polI that completely inactivates the protein? Please draw what a fork would look like in the mutant. Be sure to label your picture with the polarity of the strands and what everything is and indicate the defect. Because polI provides the exonuclease to remove the RNA primers, these will remain in the mutant. Leading strand RNA primer 3’ 5’ 5’ 3 5’ 3 5 Lagging strand Okazaki fragments 3 =...
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Homework04key - A 27 G 23 A 27 C 23 T dsDNA...

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