Homework05key

Homework05key - BIS101/Engebrecht Homework05 1. Mutants of...

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BIS101/Engebrecht Homework05 1. Mutants of Neurospora were selected on the basis of their requirement for compound B. The following data were obtained from growth tests in which the intermediate metabolites A, C, D, E, and F are supplied singly (all of the metabolites are stable and can enter the cell). + indicates growth and – indicates no growth. Accumulation of the metabolites were also measured in the mutant strains, where + = accumulation and – indicates no significant accumulation. Growth on metabolites Maximal accumulation of metabolites A B C D E F A B C D E F Mutant 1 + + - - - - - - + - - - 2 - + - - - - + - - + - - 3 + + + - - - - - - - + - 4 - + - + - - - - - - - + 5 - + - + - - - - - - - + Draw a map of the biochemical pathway, with intermediate metabolites in their proper relationship to one another, and the position of each mutation in the pathway. 3 1 E C A-- 2 B F D-- 4,5 Mutants 4 and 5 were not distinguishable from each other in the above assays. Please indicate
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Homework05key - BIS101/Engebrecht Homework05 1. Mutants of...

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