Unformatted text preview: d. Deletion of four base pairs in the middle of intron 12. 1) A deletion in the middle of the intron should have no functional consequence (normal splicing, normal gene product, normal mouse). 2) It is possible that the deletion affects the branch point sequence. In this case splicing would be abolished, resulting in a non-functional gene product. A homozygous mutant is predicted to be inviable. (Either possibility for full points) e. Mutation of the dinucleotide sequence at the 5’ end of an intron from GT to AT. Mutation of splice site results in failure to remove intron and will produce nonfunctional gene product. A homozygous mutant is predicted to be inviable. f. Deletion of the TATA sequence in the promoter. Mutation of the essential promoter element will abolish transcription of that gene. The homozygous mutant is predicted to be inviable....
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- Spring '09
- DNA, Frameshift mutation, gene product, untranslated leader sequence, nonfunctional gene product