Homework09key

Homework09key - BIS101/Engebrecht Homework09 In 8th...

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BIS101/Engebrecht Homework09 In 8 th edition: Chapter 11: 17, 21, 22, 29, 31, 34, 35; Chapter 12: 32, 35, 36 In 9 th edition: Chapter 20: 16, 18, 21, 22 1. How would you go about cloning the yeast HIS4 gene? Assume that you have a yeast his4 mutant and a genomic library that can replicate in yeast available but no sequence information. Clone gene by complementation of the mutant phenotype. To do this, transform mutant with genomic library and select for cells that can grow on medium lacking histidine. These should contain the wild-type copy of the gene that is complementing the mutant. 2. You successfully cloned the gene. You now need to analyze the DNA. Given the following information, please draw a circular restriction map of the vector and DNA fragment that represents the gene. The 5 kb vector has a single BamHI site that was used to construct the library. The vector also has a single EcoRI site but has no HindIII sites. Digestion of your DNA with BamHI generates 2 fragments: 3.5kb, 5kb
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Homework09key - BIS101/Engebrecht Homework09 In 8th...

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