Homework09key - BIS101/Engebrecht Homework09 In 8th edition...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
BIS101/Engebrecht Homework09 In 8 th edition: Chapter 11: 17, 21, 22, 29, 31, 34, 35; Chapter 12: 32, 35, 36 In 9 th edition: Chapter 20: 16, 18, 21, 22 1. How would you go about cloning the yeast HIS4 gene? Assume that you have a yeast his4 mutant and a genomic library that can replicate in yeast available but no sequence information. Clone gene by complementation of the mutant phenotype. To do this, transform mutant with genomic library and select for cells that can grow on medium lacking histidine. These should contain the wild-type copy of the gene that is complementing the mutant. 2. You successfully cloned the gene. You now need to analyze the DNA. Given the following information, please draw a circular restriction map of the vector and DNA fragment that represents the gene. The 5 kb vector has a single BamHI site that was used to construct the library. The vector also has a single EcoRI site but has no HindIII sites. Digestion of your DNA with BamHI generates 2 fragments: 3.5kb, 5kb Digestion of your DNA with EcoRI generates 3 fragments: 1kb, 2kb, 5.5kb
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern