Outline05Lectures9-10 - BIS101-001/Engebrecht Spring 2010

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BIS101-001/Engebrecht Spring 2010 Outline05Lectures9-10 : Bacterial and Viral Genetics The genetics of bacteria and viruses have been instrumental for elucidating basic cell and molecular processes. They have also been the workhorses for recombinant DNA technology as we will see later in the course. Reading in Chapter 5, 151-165 in 8 th edition or Chapter 5, 181-198 in 9 th edition. I. Properties of bacteria-see next page for nomenclature a. Prokaryote = single celled organisms that lack membrane bound organelles b. Fundamental processes are similar (e.g. DNA replication, transcription, translation) c. Mutants (nutritional markers). i. Prototrophic = can synthesize everything for self if given carbon source, inorganic salts and water ii. Auxotrophic = requires a particular nutrient d. Replica plating e. Screen vs. Selection II. Recombination in bacteria a. Conjugation = unidirectional transfer of DNA from donor to recipient, requires physical contact between cells b. Gene mapping by conjugation (
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This note was uploaded on 07/02/2010 for the course BIS BIS 101 taught by Professor Sanders during the Spring '09 term at UC Davis.

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Outline05Lectures9-10 - BIS101-001/Engebrecht Spring 2010

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