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Outline06Lectures11-12 - 2 T = A C = G 3 T A does not...

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BIS101/Engebrecht Spring10 Outline06Lectures11-12 DNA Structure and Organization in Chromosomes Reading in 8 th edition Chapter 7, 227-236, in 9 th edition, Chapter 7, 265-275 I. DNA as hereditary material We will only briefly cover this in class but I have posted a handout on the key experiments that demonstrated that DNA was the hereditary material ( Handout11DNAHereditaryMaterial ). You are responsible for this material. In particular I would like you to understand the experimental designs (with controls) and the conclusions drawn. II. Structure of DNA a. building blocks of DNA: nucleotides = the sugar, deoxyribose, phosphate group and 1 of 4 bases (e.g. deoxyadenosine 5’ monophsopahte; nucleoside = the sugar and 1 of 4 bases (e.g., deoxyadenosine); base = adenine. b. Chargaff’s rule. Measured the amount of the different nucleotides in cells. Established rules: 1. Pyrimidine (T + C) = purine (A + G)
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Unformatted text preview: 2. T = A; C = G 3. T + A does not necessarily = C + G and changes from organism to organism. 4. c. Structure (Watson and Crick, 1953) 1. Experimental approach by X-ray crystallography (Franklin and Wilkins) 2. Model building (Watson and Crick) d. Relationship between structure and function. III. Chemical composition of RNA a. Sugar = ribose b. Thymine replaced by uracil IV. Denaturation/Renaturation of DNA a. melting temperature is directly proportional to base composition. b. kinetics of renaturation is related to concentration: single vs. multi-copy genes. Reading in 8 th edition Chapter 3, 80-90; in 9 th edition Chapter 2, 33-36 V. Higher order structure of DNA a. Nucleosomes b. Nuclear architecture c. Euchromatin and heterochromatin d. Structural elements of chromosomes 1. origins of replication 2. centromeres 3. telomeres 4. single copy vs. repetitive DNA...
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Outline06Lectures11-12 - 2 T = A C = G 3 T A does not...

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