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Outline13Lecture23 - jumps III Transposable elements in...

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BIS101/Engebrecht Spring2010 Outline13Lecture23 Transposons Reading in 8 th edition Chapter 13, 423-446; in 9 th edition Chapter 14, 487-510. I. Transposons= jumping genes. Discovered by McClintock in 1944 as mutations causing phenotypes that could not be genetically mapped. II. Transposition in E. coli a. Insertion elements (IS) = a mobile piece of bacterial DNA, several hundred nucleotide pairs in length, that is capable of inactivating a gene into which it inserts. b. Inverted repeat sequences = pair of IS elements c. Mechanisms of transposition i. Replicative = copy of transposon left behind when jumps ii. Non-replicative (or conservative) = excision of transposon when
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Unformatted text preview: jumps III. Transposable elements in eukaryotes-25-40% of mammalian chromosomes consist of transposable elements that have accumulated in the course of evolution a. Retrotransposon- complex transposon with an RNA intermediate encapsulated in a specific structure called the capsid. i. LINES = long interspersed repeats ii. SINES = short interspersed repeats b. Retroviruses= retrotransposons with an extracellular stage that can infect cells c. Reverse Transcriptase= RNA transcribed to DNA IV. Applications of transposons a. Mutagenesis b. Transgenesis...
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