Outline14Lectures24-27 - BIS101/Engebrecht Spring2010...

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BIS101/Engebrecht Spring2010 Outline14Lecture 24-27 Recombinant DNA Technology and Genomics Recombinant DNA technology has transformed biology in the last 25 years. All of the techniques are the application of enzymes, processes, and paradigms we have encountered in the previous lectures. Reading in 8 th edition Chapter 11, 341-360; in 9 th edition Chapter 20, 715-740. I. Restriction enzymes = enzymes which cleave dsDNA at specific target sites (= recognition site) a. Recognition site = palindromic sequence, most common is hexameric (but tetrameric and octomeric exist) b. Biological function c. Type II restriction enzymes i. 5’ overhanging (or sticky) ends ii. 3’ overhanging (or sticky) ends iii. blunt ends d. Applications II. Isolation and cloning of DNA a. DNA extraction b. Vectors = small molecule that has a selectable marker, is capable of prolific replication and has restriction sites for insertion of DNA i. Plasmids = autonomously replicating circular DNA alpha-complementation: One of the many uses of the beta-
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This note was uploaded on 07/02/2010 for the course BIS BIS 101 taught by Professor Sanders during the Spring '09 term at UC Davis.

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Outline14Lectures24-27 - BIS101/Engebrecht Spring2010...

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