Handout25DeficiencyMapping

Handout25DeficiencyMapping - Cross 1 x mutant P AB EFGHIJKL...

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BIS101/Engebrecht Handout25DeficiencyMapping Gene order on chromosome: A . B C D E F G H I J K L Deletion stocks (heterozygous) 1) --------------( )---------------------------------------------------------------------- 2) -----------------------( )------------------------------------------------ 3) --------------------------------------------------( )------------------------ 4) ------------------------------------------------------------------------------( )----- Mutation to be mapped: ----------------------------------------------------------------h-----------------------------------
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Unformatted text preview: Cross 1 x mutant: P AB EFGHIJKL x ABCDEFGhIJKL ABCDEFGHIJKL ABCDEFGhIJKL F1 AB EFGHIJKL and ABCDEFGHIJKL ABCDEFGhIJKL ABCDEFGhIJKL Therefore, all progeny are heterozygous H/h and wild type Cross 3 x mutant: P ABCDEF JKL x ABCDEFGhIJKL ABCDEFGHIJKL ABCDEFGhIJKL F1 ABCDEF JKL and ABCDEFGHIJKL ABCDEFGhIJKL ABCDEFGhIJKL Therefore, 1/2 progeny are mutant because the recessive mutation is uncovered and now there is only the one mutant copy and 1/2 progeny are heterozygous H/h and wild type....
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