Lecture14S10

Lecture14S10 - BIS101/Engebrecht Lecture14 Genetics of Gene...

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BIS101/Engebrecht 4/30/10 Lecture14 Genetics of Gene Function: DNA makes RNA makes protein; however, while this is now considered the central dogma in biology, a lot of experimental data was collected to elucidate this. Work from Neurospora, the bread mold, was key to understanding the relationship between the gene and the protein. One gene-one enzyme hypothesis: Neurospora is a fungi that is prototrophic (meaning it can synthesis all material needed for growth from inorganic salts and a carbon source). It can be propogated as a haploid or a diploid. We discussed the importance of this alteration in life cycle with respect to isolating mutants and performing complementation tests. Beadle and Tatum combined genetics with biochemistry to formulate the one gene-one enzyme hypothesis. They isolated a number of recessive mutations in Neurospora that were auxotrophic for arginine, meaning that these mutants could not growth without added arginine to the medium. They first determined ifthe mutations were dominant or recessive by crossing them to wild type. If the resulting diploid was wild type, then the mutation was recessive. If the resulting diploid was mutant, then the mutation was dominant. Among the recessive mutations they asked how many genes the mutations represented by complementation testing. If the mutations were in the same gene, when they crossed them, the diploids were still unable to synthesis arginine (they failed to complement) but if the mutations were in different genes they were said to complement and were able to synthesis their own arginine (grow in the absence of added arginine). This analysis revealed that there were 4 complementation groups (four different genes required for the biosynthesis of arginine). They then considered the biosynthetic pathway for making arginine:
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This note was uploaded on 07/02/2010 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

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Lecture14S10 - BIS101/Engebrecht Lecture14 Genetics of Gene...

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