BacTran8 - 1. Restriction digestion of a bacterial plasmid...

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7/7/10 1. Restriction digestion of a Tips are sterile NOTE: You may need to spin some tubes in the microcentrifuge in order to get the solution at the bottom of the tube so that you may remove some with a micropipet.
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7/7/10 A. DIGESTION OF BACTERIAL PLASMIDS The digest tube should contain: 7.5 “ H2O Orange tube 2.0 “l buffer Blue tube 2.0 “ l plasmid TA provides 2.0 “ l enzyme TA provides 13.5 RlTOTAL
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BacTran8 - 1. Restriction digestion of a bacterial plasmid...

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