BIOL_208_-_FINAL_EXAM_REVIEW_2009

BIOL_208_-_FINAL_EXAM_REVIEW_2009 - BIOLOGY 208 - SPRING...

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Hybridization : - Creating an oligonucleotide probe by incorporation of  labeled nucleotides (DIG-UTP / 32P) - Creating and screening of genomic or cDNA libraries  in bacteriophage - Low and high stringency hybridization - Gel electrophoresis (agarose, SDS-PAGE,  denaturing) - Blotting (Southern, Western, Northern) Analysis of proteins : - Immunohistochemistry / Immunocytochemistry,  Generation of polyclonal antibodies in rabbits - 2-D gel electrophoresis, X-ray crystallography PCR techniques : - 5’ and 3’ RACE, RT-PCR, qPCR, PCR amplification  using - degenerate primers, Site-directed mutagenesis Characterization of cloned genes : -Cycle DNA sequencing ,  In situ  hybridization Gene Expression analyses: - Reporter probe assay, DNAse I footprinting assay,  Fusion vectors : - Transcriptional and Translational fusion vectors Post-genomic Analyses
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