Chapter_11_-_Products_from_Native_and_Ma

Chapter_11_-_Products_from_Native_and_Ma - Products from...

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Products from Native and Manipulated Cloned Genes
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11.1 Factors Affecting Expression of Cloned Genes Many factors influence the expression of a gene in bacteria Promoter site : binding of RNA polymerase depends on -35, -10 promoter consensus sequence upstream of transcriptional start site G+C content : higher G+C context of the foreign gene may hinder unwinding of the helix, hindering transcription Ribosomal binding site : Ribosomes bind to the mRNA adjacent to the start codon (Shine Dalgarno sequence) Codon usage : Some organisms have a bias for certain synonymous codons, depending on the availability and specificity of tRNA molecules that recognize these codons Post-translational cleavage or modification could be essential for biological activity, but not present in bacteria Figure 11.1 Gene expression Figure 11.2 Principal factors in bacterial gene expression
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11.2 Expression of Cloned Genes in Bacteria 11.2.1 Transcriptional fusions Expression of a foreign gene in a bacterial host requires a transcriptional fusion
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This note was uploaded on 07/10/2010 for the course BIOL 208 taught by Professor Chuong during the Spring '09 term at Waterloo.

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Chapter_11_-_Products_from_Native_and_Ma - Products from...

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