Expression_plasmids

Expression_plasmids - plasmid replication vector p p CDS...

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Unformatted text preview: plasmid replication vector p p CDS MCS LacZ bacteria Am Am Amp LacZ r - plasmid replication express Ampr r r ori ori CDNA Mammalian expression vector (Transcriptional fusion) KOZAK MCS p H BG A p bacteria Ampr - plasmid replication express Ampr CDS Am r mammalian cell SV40 ori r Neo r ori S v40 pA Neo SV 40 - plasmid replication r P express Neo - express protein acterial expression vector (Translational fusion vector) Bacteria with native RNA polymerase (DH5alpha strain RB 6x S-A Hi T s- GEK Am Amp MCS r -plasmid replication - express Ampr r p bacteria with T7 RNA polymerase (BL21 strain) ori Vector alone Ampr 6xHis-EK RB 6x S-A Hi T s- GEK - plasmid replication express Ampr - +IPTG (inducer): express 6xHis in bacteria Am CDS in reading frame r p Ampr 6xHis-EK-CDS MCS ori Vector and insert plasmid replication and express Ampr + IPTG (inducer): express 6xHisCDS fusion in bacteria ...
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This note was uploaded on 07/10/2010 for the course BIOL 208 taught by Professor Chuong during the Spring '09 term at Waterloo.

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Expression_plasmids - plasmid replication vector p p CDS...

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