nucleic_acid_purification - (spin column) solid DNA ethanol...

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DNA LIPID AAAAAA rRNA, tRNA chromosomal plasmid DNAse to eliminate DNA RNAse to eliminate RNA alkaline denaturation Homogenation, Lysozyme, EDTA, SDS Phenol-chloroform Extraction Trizol reagent (GTC-Phenol- chloroform Extraction) Oligo-dT affinity column Purified, aqueous DNA Silica-based, DNA-binding resin
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Unformatted text preview: (spin column) solid DNA ethanol precipitation aqueous mRNA solid mRNA ethanol precipitation RNA Guanidium Isothiocyanate (GTC) eliminates RNAses Extraction and Purification of Nucleic Acids in vitro PCR product or post- restriction digest ethanol precipitation...
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This note was uploaded on 07/10/2010 for the course BIOL 208 taught by Professor Chuong during the Spring '09 term at Waterloo.

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