Lecture_11_Primary_Axis_III

Lecture_11_Primary_Axis_III - OutlineofLecture10...

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Outline of Lecture 10 Longitudinal Axis Formation 1. The Role of Auxin a) Polar Auxin Transport b) Generating Polarity Mutants c) MONOPTERUS (MP) 2. Positioning the RAM
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Polar Auxin Transport In the shoot, auxin is synthesized by young  leaves and transported down stem The root has two main auxin streams (through  the stele and then back up the root through  the periphery) Fig. 4.5 From, Leyser and Day. 2003. Mechanisms in Plant Development. Blackwell Publishers.
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Onset of Polar Auxin Transport From, Leyser and Day. 2003. Mechanisms in Plant Development. Blackwell Publishers Correlates with polarized distribution of the auxin  efflux carrier, PIN1
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The role of auxin in longitudinal axis  formation Early globular Mid-globular Mid-heart Mid-globular Mid-heart Hardtke and Berleth. 1998. EMBO J. 17:1405-1411. monopteros Very early development is normal in  embryos of monopteros (from Greek  meaning “single wing”) mutants but  at maturity, seedlings lack a radicle  and some or all of the hypocotyl.
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MP and auxin 1. PIN1 polarization is normal in mp mutants 2. Mutant mp plants, however, have a significantly reduced  rate of auxin transport relative to wild type plants 3. The reduced rate of transport may therefore result in the  failure to establish a longitudinal axis 4. MP gene encodes an auxin response factor (ARF) and  hence it is likely that mp embryos are defective in auxin  signal transduction
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Auxin and polarity Loss of bilateral symmetry can be mimicked by inhibition of auxin  transport Thomas Berleth and Steve Chatfield. (2002) Embryogenesis: Pattern formation from a single cell. Sept. 30, 2002.  The Arabidopsis Book. Rockville, MD: American Society of Plant Biologists.doi 10.1199/tab.0051, http://www.aspb.org/publications/arabidopsis/
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1. HOBBIT (HBT) gene is that is required for RAM formation 2. Strong hbt mutant alleles completely lack a RAM   Willemsen et al, 1998. Development  125 :521-531. strong
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This note was uploaded on 07/10/2010 for the course BIOL 321 taught by Professor Susanlolle during the Winter '09 term at Waterloo.

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Lecture_11_Primary_Axis_III - OutlineofLecture10...

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