Lecture_6_Chimeras

Lecture_6_Chimeras - Re w of Le vie cture5 1. Plasm sm ode...

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Review of Lecture 5 1. Plasmodesmata Selective trafficking Movement of non-cell autonomous proteins (NCAPs) Symplastic domains 2. Lineage markers and cell autonomy 3. Determining lineage relationships using chimeras
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http://www.tulane.edu/~dmsander/WWW/335/pdtitle.gif pegacat.com/cbetts/ phd/cellintro/cellintro.html Hypothetical 3D Structure Actin Plasma Membrane Proteinaceous struts desmotubule
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From Kurata et al. 2005. Current Opinion in Plant Biology. 8 :600-605 KNOTTED1 was the first transcription factor shown to move through PD and demonstrated to facilitate its own RNA movement
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http://jacksonlab.cshl.edu/three.html Micrographs showing STM transcription factor movement from L1 to L2
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Implications for Development Symplastic compartment : physiological pH, standard sources for cellular energy, organized macromolecular structures and interactions, asymmetric, developmentally fluid, similarities with other cellular systems Apoplastic compartment : more acidic (pH 4-5), inert biopolymer composition, molecular repository, great diversity in molecular composition, asymmetric, developmentally fluid, cohabitation with other organisms Both domains must retain function over wide temperature fluctuations and adapt accordingly
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Lineage Relationships in Plants To understand the relationship between cell lineage and position, cells must be marked at an early stage in development and the cells derived from this marked population of cells followed over time The marker must be a visible and stable heritable marker (it must be cell-autonomous )
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Cell-Autonomous Markers Useful plant cell-autonomous markers include, 1. pigments (plastids) 2. nuclear ploidy levels 3. specialized cell types (hair cells) http://faculty.ucc.edu/biology-ombrello/Images/varieg%20pothos.jpg SEM of developing Arabidopsis leaf showing trichomes
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Lecture 6 Outline Lineage relations Clonal analysis using graft chimeras and sector analyses 1. Fate of cells derived from each of the three meristematic layers a) History b) Classical studies using polyploids in Datura
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Graft Chimeras 1. History 2. The Bizzarria Orange 3. Classical Studies on Datura by Satina, Blakeslee and Avery in the 1940s
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This note was uploaded on 07/10/2010 for the course BIOL 321 taught by Professor Susanlolle during the Winter '09 term at Waterloo.

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Lecture_6_Chimeras - Re w of Le vie cture5 1. Plasm sm ode...

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