Lecture_7_Chimeras_II

Lecture_7_Chimeras_II - ReviewofLecture6 1....

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Review of Lecture 6 1. The history of grafting 2. The chimera concept 3. Classical studies of Satina, Blakeslee and Avery  using Datura The three germ layers Lineage in different meristem types Lineage relationships in lateral organs
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The Bizzarria Orange Resulted from grafting a sour orange scion to a citron stock.  A bud arose at the graft  junction with a unique mix of parental characteristics. http://www.homecitrusgrowers.co.uk/citrusvarieties/bizzarria.html
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Vegetative Propagation  Shoot grows out from callus tissue scion stock callus
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Chimera Concept From R.A.E. Tilney-Bassett. Plant Chimeras. 1986. Arnold Publishers
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Convention Periclinal chimeras are described according to  the relative contribution of graft  partners, starting with the L1 1. If  plant A  is grafted to  plant B  and a  periclinal chimera is produced, the layer  contributions are listed beginning with  the L1, then the L2 and finally the L3 2. ABA: L1 is A, L2 is B, L3 is A 3. BBA: L1 is B, L2 is B, L3 is A L1 L2 L3 SAM
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The Debate in 1940s Datura Periclinal Chimeras 1. Focusing on the question of the evolutionary origin of  flowers 2. Are flowers novel structures or are they modified pre- existing structures? 3. Is the floral apex a modified vegetative apex? 4. Are floral organs modified leaves?  
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From Satina and Blakeslee, 1941. Am. J. Bot. 28:862-871. Vegetative and Floral Apices (L1,L2,L3)
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What about floral organs? Are floral primordia modified leaf primordia? From Satina and Blakeslee, 1941. Am. J. Bot. 28:862-871. Periclinal divisions in L2 L3 Procambial tissue  The bulk of the leaf tissue is L2 derived
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Stamens and Carpels Leaf pattern is modified From Satina and Blakeslee, 1941. Am. J. Bot. 28:862-871. 1. The bulk of the organ tissue is L3 derived 2. No periclinal divisions are seen in the L2 3. L1 and L2 contribute to surface growth 4. Only late in development do L2 cells predominate (in  anthers) 5. Gametes are derived from the L2
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Lecture 7 Outline Lineage Relationships 1. Sectorial Chimeras 2. Definitions 3. Green-white-green (GWG) periclinal chimeras  4. Laser Ablation Experiments 5. What have we learned?
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Using cell autonomous markers to determine lineage Within Organs At the SAM
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This note was uploaded on 07/10/2010 for the course BIOL 321 taught by Professor Susanlolle during the Winter '09 term at Waterloo.

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Lecture_7_Chimeras_II - ReviewofLecture6 1....

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