Fulltext25 - Fluorescent Probes and Labels for Biomedical Applications LEONID PATSENKER,a,b ANATOLIY TATARETS,a OLGA KOLOSOVA,a OLENA OBUKHOVA,a

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Fluorescent Probes and Labels for Biomedical Applications L EONID P ATSENKER , a , b A NATOLIY T ATARETS , a O LGA K OLOSOVA , a O LENA O BUKHOVA , a Y EVGENIY P OVROZIN , a I RYNA F EDYUNYAYEVA , a I NNA Y ERMOLENKO , a AND E WALD T ERPETSCHNIG b a State Scientific Institution “Institute for Single Crystals” of The National Academy of Sciences of Ukraine, Kharkov, Ukraine b SETA BioMedicals, Urbana, Illinois, USA Fluorescence probes and labels have become indispensable tools for clinical diagnostics, high- throughput screening, and other biomedical applications. We have developed several classes of new squaraine-based red and near-infrared (NIR) probes and labels (SETA and Square series), naphthalimide-based fluorescence lifetime dyes (SeTau series), and cyanine- and squaraine-based quenchers (SQ series). This report discusses the spectral and photophysical properties of these new markers. In particular, the red and NIR dyes of the SETA and Square series are extremely bright, with photostabilities that are unmatched by any other dyes in the same spectral region. Key words: biomedical probes and labels; quenchers; lifetime labels; pH-sensitive dyes; biological imaging Introduction Fluorescent but also nonfluorescent dyes (quenchers) are used for a wide range of biomedical applications. Whereas fluorescent labels are used for covalent attach- ment to biological species, fluorescent probes undergo noncovalent interactions with a biomolecule or the an- alyte of interest. Despite the many commercially avail- able fluorescent dyes, they still suffer from shortcom- ings, such as low fluorescence quantum yields (QYs) in aqueous media and insufficient photostability. Also, there is a need for not only brightly fluorescent dyes but also truly dark quenchers for energy transfer measure- ments that exhibit no residual fluorescence. In particu- lar, in the red and near-infrared (NIR) region, the lack of bright, photostable markers for imaging applications is still pronounced. Recently we reported the synthesis, spectral prop- erties, and biomedical applications of squaraines and ring-substituted squaraines (Square and SETA dyes). 1–6 Here we describe new probes and labels and compare them with other commercially avail- able markers. Meanwhile we introduce the new SeTau Address for correspondence: Leonid Patsenker, State Scientific Insti- tution “Institute for Single Crystals” of the NAS of Ukraine, 60 Lenin Ave., 61001 Kharkov, Ukraine. Voice: + 38 057 3410272; fax: + 38 057 3409343. [email protected] and SQ series of dyes, which are represented by fused aromatic compounds and cyanine dyes, respectively. These are a series of extremely bright and photostable fluorescence dyes and dark quenchers for use in bio- logical and pharmaceutical research, clinical diagnos- tics, and high-throughput screening. These materials include reactive red and NIR fluorescent labels of the Square and SETA series for covalent attachment to biomolecules, such as proteins, amino acids,
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This note was uploaded on 07/11/2010 for the course SPECTOGRAP 545 taught by Professor Gdf during the Spring '10 term at AIB College of Business.

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Fulltext25 - Fluorescent Probes and Labels for Biomedical Applications LEONID PATSENKER,a,b ANATOLIY TATARETS,a OLGA KOLOSOVA,a OLENA OBUKHOVA,a

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