Chapter 15 – DNA Technology and Genomics

Chapter 15 – DNA Technology and Genomics -...

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Chapter 15 – DNA Technology and Genomics I) DNA Cloning a. History of Recombinant DNA Technology i. Developed first during the 1940’s using bacteria and bacteriophages b. Usage of Recombinant DNA Technology i. Restriction enzymes cut DNA into fragments 1. Enzymes from bacteria that are used to cut DNA molecules at specific places ii. DNA fragments are then inserted into a suitable vector
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Unformatted text preview: 1. Vector a. Carrier capable of transporting the DNA fragment into a cell iii. The plasmid of the DNA fragments are then inserted into the bacterial cells by transformation (take up of foreign DNA fragmets) 1. Researcher alters the cells chemically or by electroporation (electric shock) to make plasma membrane permeable to the plasmid DNA molecules c....
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This note was uploaded on 07/17/2010 for the course 119:102 119 taught by Professor Uzwiak during the Spring '09 term at Rutgers.

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