Directed evolution

Directed evolution - Enzyme engineering Increasingly used...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
Enzyme engineering Increasingly used for manufacture of products until now derived using traditional chemical processes Pharmaceutical and agrochemical intermediates Fine and bulk chemicals biofuels Need to improve enzymes for effectiveness in industrial conditions
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Desirable properties High catalytic turnover (kcat>500/min) High selectivity for reaction High process stability under industrial conditions (temperature, pH, organic solvents, detergents) Sustainable processes High selectivity, low specificity A -> A-P B -> B-P C -> C-P :
Background image of page 2
Industrial applications > 500 products covering 50 applications from detergents to beer making utilized enzymes Used in detergent, starch, textile, leather and personal care Food enzymes (25 %), includes enzymes in dairy, brewing, wine and juice, fats and oils Feed enzymes used in animal feeds Current Opinion in Biotechnology 2003, 14:438–443
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Advantages of enzymes over chemical catalysts Derived from renewable resources
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 07/19/2010 for the course BME 3604 taught by Professor Lt during the Spring '10 term at University of Florida.

Page1 / 16

Directed evolution - Enzyme engineering Increasingly used...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online