{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lecture 4 - to the amide nitrogen of asparagine side chains...

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Mon. 8.10.09 Lecture 4: Regulation of Protein Function & Protein Analysis I. Post-Translational Protein Modifications Protein acetylation o Addition of acetyl group (CH3CO) to the amino group of the N-terminus residue. o Plays an important role in controlling the life span of proteins within cells. Hydroxylation o Hydroxyl groups (OH) can be added to lysine or proline. o Proline is the principal residue to be hydroxylated in proteins, which occurs at the delta-C atom, forming hydroxyproline, and essential element of collagen, in turn a necessary element of connective tissue. Glycosylation o Addition of saccharides to membrane and secreted proteins that contain asparagine, serine or threonine. o The majority of proteins synthesized in the rough ER undergo glycosylation. o Glycosylated proteins are normally found on the cell surface embedded within the plasma membrane. o 2 types: N- linked glycosylation:
Image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: to the amide nitrogen of asparagine side chains. O-linked glycosylation: to the hydroxyl oxygen of serine and threonine side chains. • More for regulation o Glycosylation serves various functions: Some proteins do not fold correctly unless they are glycosylated first. Polysaccharides linked at the amide of nitrogen of asparagine confers stability of some secreted glycoproteins… where unglycosylated proteins are quickly degraded. Cell adhesion. • A protein may be anchored to the membrane if it is attached with lipid molecules. II. Regulation of Protein Function • Regulation of protein activity by kinase / phosphatase switch. o Phosphorylation changes a protein’s charge and generally leades to a conformational change—which may lead to activation or inactivation of the protein function. o Ooooooo used in the kinase/phosphatase switch....
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern