E-TLC - Elizabeth Doane Thin Layer Chromatography...

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Elizabeth Doane Thin Layer Chromatography Discussions and Conclusions Features of a good chromatography analysis: There are many different features of a good TLC analysis. But first, in order to achieve a good TLC analysis, the plate must be properly prepared. The plates need to be handled with care so that no coating flakes off (the coating is silica or alumina and is very thin). To prepare the plates, a thin pencil mark should be drawn across the end to ensure that all solutes begin in approximately the same place. When spotting the solutes onto the plate there should not be too much or too little. After it has developed a good TLC should have clear runs without much streaking, and a light pencil mark should be made at the solvent front. Polar v. Non-Polar in regard to chromatography : During the TLC, I observed the more non-polar a substance was, the farther it moved up on the plate. For example, Fluorene, a non-polar substance, moved the farther up than did the polar substance, 9 fluorenon. Also, more separation occurred when
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This document was uploaded on 07/23/2010.

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