Lab 7 - Lab 7: Thin Layer Chromatography (TLC) Purpose: The...

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Lab 7: Thin Layer Chromatography (TLC) Purpose: The purpose of this lab is to use Thin Layer Chromatography (TLC) to identify the components in an unknown analgesic. Discussion TLC is used to test a compound’s preference over a stationary or mobile phase. A layer of solid silica gel plate serves as the adsorbent in the stationary phase while the solvents are used as the mobile phase. Based on their intermolecular forces, the compounds being examined would prefer either the silica or the solvent; the stronger the interaction between a component and the stationary phase, the slower the component is moved by the mobile phase, vice versa. A good TLC solvent give the best separation for the known compounds: each compound can be easily distinguished from others and hence their Rf values are unique. As a result of the experiment, 100% ethyl acetate and 98:8 ethyl acetate: acetic acid are chosen as our solvents. An unknown pain killer and four pure analgesic components are spotted on two plates for the two chosen solvents for comparison: the components of the unknown can be determined since they respond differently under different mobile phases. The
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This note was uploaded on 07/23/2010 for the course CHEM Chemistry taught by Professor Nitche during the Spring '08 term at Berkeley.

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Lab 7 - Lab 7: Thin Layer Chromatography (TLC) Purpose: The...

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