Restriction Enzymes

Restriction Enzymes - 1.Cloningagene 1.HumanGene = 2. 3. DNA RestrictionEnzymes

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Recombinant DNA technology
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1. Cloning a gene 1. Human Gene + Bacterial Plasmid vector = Recombinant DNA molecule 2. Introduction of recombinant DNA  molecule into bacteria 3. Selection of bacteria with recombinant  DNA
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Restriction Enzymes First discovered by Hamilton Smith at Johns Hopkins in  1970 Bacterium Haemophilus influenzae rapidly broke  down viral DNA The enzyme broke down E coli DNA but it did not cut  DNA from Hameophilus influenzae Called  Hin dII, it binds specifically to the following  sequences in DNA  (5‘) G T ( pyrimidine: T or C) ( purine: A or G) A C  (3‘)   (3‘) C A ( purine: A or G) ( pyrimidine: T or C) T  G (5’)
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Sticky ends From Alberts, Molecular  Biology of the Ce
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Restriction Enzymes Typically recognize 4 to 8 base pair  sequences called restriction sites and  cleave both DNA strands Called restriction endonucleases as  opposed to exonucleases
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This note was uploaded on 07/23/2010 for the course BME 3406 taught by Professor Lele during the Spring '10 term at University of Florida.

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Restriction Enzymes - 1.Cloningagene 1.HumanGene = 2. 3. DNA RestrictionEnzymes

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