PCR - Cloning genes Cloning results in the purification of...

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Cloning genes Cloning results in the purification of a single fragment of DNA from exceedingly complex DNA molecules. Need DNA corresponding to the gene. Restriction enzymes do not produce genes, but DNA fragments that may or may not contain the gene Need to separate gene of interest from DNA For example, the β-globin gene comprises only 0.00005% of the 3300 megabases (Mb) of human genomic DNA, and even the massive 2.5 Mb dystrophin gene, the largest gene that has been identified, accounts for only about 0.08% of human genomic DNA.
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DNA Polymerase: template dependent synthesis of DNA From Genomes 2, by TA Brown DNA polymerase
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http://www.dnalc.org/resources/animations/sangerseq.html Reminder: chemistry of DNA replication
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The requirement of a primer is extremely useful From Genomes 2, by TA Brown
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DNA polymerase also has exonuclease activity From Genomes 2, by TA Brown
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PCR - Cloning genes Cloning results in the purification of...

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