07 - prot-ligand

07 - prot-ligand - 3 / 1 1 / 2 0 1 0 1 Protein...

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Unformatted text preview: 3 / 1 1 / 2 0 1 0 1 Protein Protein-ligand ligand interactions: interactions: human CD38 human CD38 Outline • Enzyme & ligand • CD38 structure determination Complex structures of human CD38 with its • Complex structures of human CD38 with its substrates, products, and intermediates Enzymatic reaction pathway • Pre-transition state (Michaelis complex) Transition states (intermediates (Enzymes: biological molecules that possess catalytic activity) • Transition states (intermediates) insights into mechanisms drug design (e.g. Tamiflu) • Products Structural tools to study protein-ligand interactions Slowdown, Freeze, Capture Inactive mutant substrate analog (inactive or “pause”) Incubating enzyme and substrate: temperature, time concentration pH product inhibition time, concentration, pH, product inhibition Short exposure/readout time Laue x-rays (wide bandwidth) Time-resolved (Multiple copies in crystallographic a.s.u) Spatial-resolved 3 / 1 1 / 2 0 1 0 2 CD38: antigen & ectoenzyme • Antigens - substances that are recognized by the immune system and induce an...
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This note was uploaded on 07/29/2010 for the course BIOC BIOC2811 taught by Professor Dr.q.hao during the Summer '10 term at HKU.

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07 - prot-ligand - 3 / 1 1 / 2 0 1 0 1 Protein...

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