Lab 4.docx - Jeremy Sousa 0585514 BIOL 2070 W03 Partner...

This preview shows page 1 - 4 out of 5 pages.

Jeremy Sousa0585514BIOL 2070 - W0303/12/2019Partner: Matthew SmithLab 4 - PROTEIN QUANTIFICATION USING THE BRADFORD ASSAY
Part 1: Tables and CalculationsTable 1 (7 marks):12345678LabelBSA concentration(µg/ml)Volume ofstock BSA(µl)Volume of0.15N NaCl (µl)Total Volume (µl)A595 nmTube Set 1A595 nmTube Set 2A595 nmAverageTube 100100100000Tube 2255951000.060.020.04Tube 35010901000.100.080.09Tube 47515851000.170.210.19Tube 510020801000.190.230.21Table 1 Calculations (2 marks):Column 3:Tube2:V1=C2V2C1=(25000µg/µ L)(100µ L)500000µ L=5µLColumn 4:Tube2:V0.15N NaCl=VTotalVStock BSA=100µL5µ L=95µ LTable 2 (6 marks):12345LabelA595 nm Protein concentration in diluted sample (µg/ml)Dilution FactorProtein concentration in undiluted sample (µg/ml)Tube 70.1777.391007739Tube 80.2090.439043Average protein concentration in Dictyosteliumsample (µg/ml)8391Table 2 Calculations (3 marks):Column 3:Tube7:y=0.0023x0.0080.17=0.0023x0.008x=(0.17+0.008)0.0023x=77.39Column 4:DF=Final volumeAliquotVolume=200µL2µL=100Table 3 (2 marks):
123LabelAmount of protein (µg)Volume of unknown solution (µl)Dicty-550.5959Dicty-10

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture