DNA Extraction

DNA Extraction - DNA Extraction Cell Lysis - Resuspend in...

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DNA Extraction Cell Lysis - Resuspend in 500µL 1xTE - Add 25µL 10% SDS and 5µL 100mg/mL ProK - Vortex, Incubate at 55°C for 1 hour - Vortex for 10 sec - Spin @ 10kg for 30 sec - Collect supernatant ~530µL DNA Extraction (in hood) - Add 1 vol chloroform/isoamyl alcohol – 500µL - Invert to mix, spin 10kg for 5 min - Transfer supernatant to fresh tube - Add 1 vol phenol/ chloroform/isoamyl alcohol –from bottom later – 400µL - Invert to mix, spin 10kg for 5 min - Transfer supernatant to fresh tube Ethanol Precipitation
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This note was uploaded on 08/06/2010 for the course PHYSICS 1021 taught by Professor Tolly during the Spring '10 term at Acadia.

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DNA Extraction - DNA Extraction Cell Lysis - Resuspend in...

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