DNA Extraction - DNA Extraction Cell Lysis Resuspend in 500L 1xTE Add 25L 10 SDS and 5L 100mg\/mL ProK Vortex Incubate at 55C for 1 hour Vortex for 10

DNA Extraction - DNA Extraction Cell Lysis Resuspend in...

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DNA Extraction Cell Lysis - Resuspend in 500µL 1xTE - Add 25µL 10% SDS and 5µL 100mg/mL ProK - Vortex, Incubate at 55°C for 1 hour - Vortex for 10 sec - Spin @ 10kg for 30 sec - Collect supernatant ~530µL DNA Extraction (in hood) - Add 1 vol chloroform/isoamyl alcohol – 500µL - Invert to mix, spin 10kg for 5 min - Transfer supernatant to fresh tube - Add 1 vol phenol/ chloroform/isoamyl alcohol –from bottom later – 400µL - Invert to mix, spin 10kg for 5 min - Transfer supernatant to fresh tube Ethanol Precipitation
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